Fig. 6.

The disruption of CD44–HA binding altered the metabolic and proliferative state of cancer cells. Metabolic activity of (A) U87-MG, (B) A375 and (C) HT29 cancer cells challenged to HA-coated plates was assessed with resazurin for 24 h after treatment. Cell, HA-coated plates or both were treated with CAP (A-C: 50 Hz left; 100 Hz right). Metabolic activity was assessed with resazurin for 24 h after treatment. Wells coated only with BSA (no HA) served as negative controls. ΔOD = optical density corrected for background. Cell proliferation was assessed by measuring the confluence of the cultures over 72 h for (D) U87-MG, (E) A375 and (F) HT29 cancer cells. (D–F) Data is expressed as confluency normalized to T = 0 h. Statistically significant differences were found between the sham-treated (ST) control and corresponding treated samples. Mean ± S.D. * = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001; **** = p ≤ 0.0001. In all cases, data were analyzed using the Dunnett post hoc test.