Skip to main content
. 2021 Feb 26;21(2):e13. doi: 10.4110/in.2021.21.e13

Figure 3.

Figure 3

CD11b-defieicnt macrophages produce increased amounts of pro-inflammatory mediators. (A) BMDMs were treated with MRSA for 8 h, and cytokine levels in the culture supernatants were measured using the Cytokine Bead Array. (B) iNOS expression was assessed by immunoblotting and intensity of iNOS signal in each band was normalized by β-actin. Shown is the representative of 3 independent experiments. (C) NO production was measured as nitrate levels using the Griess reagent. (D) Expression of mRNA of the indicated cytokines was analyzed by quantitative real-time RT-PCR. Concentration of each mRNA was normalized to Actb, and depicted as the relative expression levels to those of the control macrophages. Data are presented the mean (±SEM) from 2 independent experiments with 3 biological replicates. (E-G) Peritoneal macrophages were treated with MRSA for 8 h, and cytokine levels (E), NO production (F) and mRNA expression (G) were determined as described above. (H) BMDMs were treated with MRSA for 8 h, and analyzed by flow cytometry. Shown are representative FACS profiles with frequencies of indicated subsets in the viable TCRβ− B220− F4/80+ gates.