Figure 5.

Increased activation of NF-κB signaling and phosphorylation of Akt in CD11b-deficient macrophages. (A) BMDMs were activated with LPS for the indicated times, and analyzed by Western blotting. Representative results from 2 or 3 independent experiments are shown. Immunoblotting of α-tubulin and unphosphorylated proteins was used as a loading control. (B) Accelerated nuclear induction of NF-κB p65 in CD11b-deficient macrophages. BMDMs were treated with LPS for the indicated times, and stained using anti-p65, followed by Alexa Fluor 488-conjugated secondary antibody. Shown are representative confocal images obtained from 3 independent experiments. DAPI (blue) was used to stain nucleus. (C) BMDMs were treated with IL-4, and phosphorylation of STAT6 and Akt was analyzed as in (A). (D) mRNA expression of Irf4 was measured quantitative real-time RT-PCR 8 h after IL-4 treatment. Data are presented the mean (±SEM) from 2 independent experiments with 3 biological replicates.