Table 1. List and structures of the promoters tested with the chloramphenicol acetyltransferase (CAT) assay.
| Genes | -35 box | Spacer (length [bp]) | -10 box |
|---|---|---|---|
| groES (BL1558) | TTGGCA | GAGTGCTAATAATCGCGC (18) | TACGAT |
| gap (BL1363) | TTGCCA | TGTGTACAGAGTCGGCAT (18) | TACAGT |
| rpmB (BL0330) | TTGCGG | TTGTCGGTATTGGGCTA (17) | TATATT |
| rpmH (BL0642) | TTGACT | TGAGGGTAGGGGTGAGAG (18) | TACTTT |
| BLt43 | TTGCGA | TAATCCACCGGAGCCGT (17) | TACTAT |
| rplU (BL1282) | TTGATT | TAGTCCGCATGGTGGTG (17) | TAGATT |
| tuf (BL1097) | GTGGCA | CATAACCCAGAAACCCAG (18) | TAGAAT |
| rplM (BL1571) | TTGCCC | TATGTCAAGCCCGGCGA (17) | TATACT |
| BL1230 | TTGTGA | TTCAAATTATCATTCAT (17) | TACAAT |
| hup (BL1798) | TTCGCA | GAAACATGCGC (11) | TAGTAT |
| BL1769 | TTGACA | ACATTGGTAAAAGGTTA (17) | TATCAT |
The target promoters of the CAT assay were the upstream regions of the listed genes. We also showed the promoter core motifs, termed -35 and -10 boxes, and the sequences of the spacers.