Figure 4.
Overexpression of G6pc decreased glycogen accumulation in MED13-LKO. A)G6pc gene expression in both Med13fl/fl and MED13-LKO mice liver was measured by qRT-PCR 10 days after AAV-Tbg-GFP or AAV-Tbg-G6pc injections. B) Hepatic glycogen measurement, C) H & E staining and PAS staining in liver sections and hepatic glycogen levels, D) changes in serum glucose levels, and E) % changes in G6pc activity (n = 6) were measured as described in Methods (n = 8 per group). Identical letters indicate values that are not statistically different from each other (p > 0.05). One-way ANOVA followed by the Turkey-Kramer multiple comparisons test.