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Immunohistochemistry assay of osteogenic markers for composite scaffold materials. BMSCs were cultured on different scaffold materials for 7 days and next on slides for 3 days. They were subjected to immunohistochemistry assay of the expression of ALP (A), OCN (B) and OPN (C). The negative control was added with PBS instead of primary antibody (D). Black bar represents 100 μm.
