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Q-PCR and Western-Blot assay of osteogenic markers for composite scaffold materials. BMSCs were cultured on different scaffold materials for 14 days. RNA was isolated and subjected to qPCR analysis of the expression of OCN and COL1α1 (A) in osteoblast cells. The expression of COL1α1 was detected by Western-Blot (B, C). “*” means P < 0.05.
