Figure 1.

Adult mouse distal colon myenteric plexus RNA-seq defines neuron and glia subtypes. (A–D) H2B-mCherry fluorescence (red) in 50-day-old Wnt1-cre^cre/wt; R26R-H2B-mCherry^ch/wt mice colocalizes with HuC/D+ neurons (green) and S100β+ glia (blue) in the ENS. ∼73% of HuC/D+ neurons were H2B-mCherry+. Scale bar = 100 μm. (E) RNA-seq workflow with t-SNE containing all cells. (F) Feature plots for Actg2, Kit, Pdgfrα, Plp1, and Elavl4 indicate the locations of SMC, PDGFRα+ cells, ICC, neurons, and glia, respectively. Color key represents log_e(normalized gene expression). (G) Violin plots of neuronal and glial clusters. (H) t-SNE of neuron clusters (I) Feature plots for selected markers highlight neuron subtypes. Color shows log_e(normalized gene expression).