Figure 16.

Human single-nucleus RNA-seq analysis from 20,167 cells yielded data from many cells that impact bowel motility including SMC, ICC, PDGFRA+ cells, muscularis macrophage, and glia. (A) Human myenteric plexus after incubation with 4-Di-2-Asp (4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide), with muscle layers partially peeled away. Scale bar = 50 μm. (B) RNA-seq workflow for adult human colon myenteric plexus. (C) t-SNE plot of 20,167 nuclei shows glia, ICC, muscularis macrophages, PDGFRA+ cells, smooth muscle, T cells, endothelium, and unknown groups. Neurons comprise 1 small cluster (∼48 cells). (D–L) Feature plots showing genes expressed highly in (D, E) adult human smooth muscle (ACTG2, MYH11), (F) vessel endothelial cells (VWF), (G, H) glial cells (PLP1, SOX10), (I) ICC (ANO1), (J) PDGFRA+ cells (PDGFRA), (K) muscularis macrophages (CD14), and (L) T cells (CD2). Color key represents log_e(normalized gene expression).