TABLE 1.
Primer sequences for RT-PCR and quantitative real-time PCR1
| Gene | GenBank accession number | Assay ID |
|---|---|---|
| Bmal1 | NM_007489.4 | Mm00500226_m1 |
| Per1 | NM_011065.4 | Mm00501813_m1 |
| Per2 | NM_011066.3 | Mm00478113_m1 |
| Cry1 | NM_007771.3 | Mm00514392_m1 |
| Krt7 | NM_033073.3 | Mm00466676_m1 |
| Acta2 | NM_033073.3 | Mm00466676_m1 |
| Rplp0 | NM_007475.5 | Mm00725448_s1 |
| Gene | Forward primers | Reverse primers |
| Bmal1 | CCTAATTCTCAGGGCAGCAGAT | TCCAGTCTTGGCATCAATGAGT |
| Per1 | GTGTCGTGATTAAATTAGTCAG | ACCACTCATGTCTGGGCC |
| Per2 | GCGGATGCTCGTGGAATCTT | GCTCCTTCAGGGTCCTTATC |
| Cry1 | TTGCCTGTTTCCTGACTCGT | GACAGCCACATCCAACTTCC |
| Krt7 | CGCCGCTGAGTGTGGACATCG | CTGGCTGCTCTTGGCTGACTTCTG |
| Acta2 | GAGGCACCACTGAACCCTA | CATCTCCAGAGTCCAGCACA |
| Rplp0 | CTCTCGCTTTCTGGAGGGTG | TCAGTCTCCACAGACAATGCC |
| Gapdh | CATGGCCTTCCGTGTTCCT | CCTGCTTCACCACCTTCTTGA |
1
Acta2, smooth muscle marker αSMA; Bmal1, brain and muscle aryl hydrocarbon receptor nuclear translocator-like protein 1; CK-7, cytokeratin 7; Cry1, cryptochrome 1; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; Krt7, endometrial epithelial marker CK-7; Per, period circadian clock; Rplp0, ribosomal protein lateral stalk subunit P0; αSMA, α smooth muscle actin.