Fig. 2.

Enzymatic activity of WT and mutant laccases against non-phenolic ABTS (A) and phenolic 2,6-DMP (B) at different pH (x axis). The average of at least 3 repetitions is shown; the error bars are the standard deviation. WT laccase is in black; mutants carrying the D206N substitution are in blue, those with a larger binding pocket are in red. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)