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. 2021 Apr 7;593(7857):101–107. doi: 10.1038/s41586-021-03420-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, Schematic showing the composition of the CHM13 chromosome 8 centromere. The centromeric region consists of a 2.08-Mb D8Z2 α-satellite HOR array flanked by regions of monomeric and/or divergent α-satellite interspersed with retrotransposons, β-satellite and γ-satellite. The predicted restriction digest pattern is shown. The D8Z2 α-satellite HOR array is heavily methylated except for a 73-kb hypomethylated region, which is contained within a 632-kb CENP-A chromatin domain (Extended Data Fig. 9, Supplementary Fig. 8). A pairwise sequence identity heat map indicates that the centromere is composed of five distinct evolutionary layers (dashed arrows). b, Pulsed-field gel Southern blot of CHM13 DNA confirms the structure and organization of the chromosome 8 centromeric HOR array. Left, ethidium bromide (EtBr) staining; right, ³²P-labelled chromosome 8 α-satellite-specific probe. n = 2. See Supplementary Fig. 9a, b for gel source data. c, Representative images of a CHM13 chromatin fibre showing CENP-A enrichment in an unmethylated region. n = 3. Scale bar, 1 μm.