Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 7;593(7857):101–107. doi: 10.1038/s41586-021-03420-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — a, Schematic showing the composition of the CHM13 8q21.2 VNTR. This VNTR consists of 67 full and 7 partial 12.192-kb repeats that span 863 kb in total. The predicted restriction digest pattern is indicated. Each repeat is methylated within a 3-kb region and hypomethylated within the rest of the sequence. Mapping of CENP-A ChIP–seq data from the chromosome 8 neodicentric cell line known as MS4221^24,25 (Methods) reveals that approximately 98% of CENP-A chromatin is located within the hypomethylated portion of the repeat. A pairwise sequence identity heat map across the region indicates a mirrored symmetry within a single layer, consistent with the evolutionarily young status of the tandem repeat. b, Pulsed-field gel Southern blot of CHM13 DNA digested with BmgBI confirms the size and organization of the chromosome 8q21.2 VNTR. Left, ethidium bromide staining; right, ³²P-labelled chromosome 8q21.2-specific probe. For gel source data, see Supplementary Fig. 1c, d. c, Copy number of the 8q21 repeat (chr8:85792897−85805090 in GRCh38) throughout the human population. CHM13 is estimated to have 144 total copies of the 8q21 repeat, or 72 copies per haplotype, whereas GRCh38 only has 26 copies (red data points). Median ± s.d. is shown.