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. 2021 Apr 20;24(5):102408. doi: 10.1016/j.isci.2021.102408

Figure 3.

Figure 3

Nanopore experiments

(A) Schematics depicting the translocation of a DNA through a nanopore.

(B) Typical blockade events for HT DNA construct passing through a nanopore.

(C) Typical nanopore traces for the HT + FIS & H-NS samples (1ng/μl final DNA concentration).

(D) Typical nanopore traces for HH + FIS & H-NS samples (1ng/μl final DNA concentration). Upon the protein addition, the blockades become deeper, indicative of protein binding. The blockade events for the HH + FIS & H-NS samples are uniform and homogeneous, indicating that the DNA-protein complexes are similarly organized.

Normalized blockade events for the (E) HT and (F) HH constructs, showing that the deeper events occur much more frequently for the HT construct (in 38% of total events, NHT+ Protein = 277 out of 737 total events), but not for the HH construct (in 18% of total events, NHH+Protein = 329 out of 1812 total events). Errors bars on panels (E) and (F) indicate five times the rms of the signal noise.