Table 2.

qPCR conditions and primer sets.

Gene	Pre incubation	Amplification	Melting curve
Ovine ATPase and IL1 beta	95 °C for 15 min	50 cycles of 94 °C 15 s 52 °C 30 s 72 °C 30 s 80 °C 5 s	95 °C 5 s ramp 4.4 40 °C 1 min ramp 2.2 97 °C continuous 0.11 72 °C 10 min ramp 2.2
Ovine TGF beta	50 °C 2 min 95 °C 8.5 min	40 cycles of 95 °C 15 s 60 °C 1 min	95 °C 1 min ramp 4.4 55 °C 1 min ramp 2.2 97 °C continuous 0.11
Ovine IL8	45 °C 30 min   95 °C 10 min	40 cycles of 94 °C 20 s 55 °C 30 s 72 °C 30 s	95 °C 5 s ramp 4.4 55 °C 1 min 2.2 95 °C continuous 0.29

	Forward 5′–3′	Reverse 5′–3′
Ovine ATPase Ovine	GCTGACTTGGTCATCTGC	CAGGTAGGTTTGAGGGGATAC
TGF beta	CTGAGCCAGAGGCGGACTAC	TGCCGTATTCCACCATTAGCA
Ovine IL1 beta	CCCATTAATGAAGTGATGGC	CTAGGGAGAGAGGGTTTCCA
Ovine IL8	CACTGCGAAAATTCAGAAATCATTGTTA	CTTCAAAAATGCCTGCACAACCTTC