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. 2021 May 5;12:2537. doi: 10.1038/s41467-021-22755-3

Fig. 2. Salmonella YB1 treatment interferes with survival and early colonization of newly deposited cancer cells.

Fig. 2

a On day 0, 4T1 cells were implanted into the fat pad of BALB/c mice. Two groups of mice were treated with YB1 on day 7 (YB1_D7, n = 10) or 12 (YB1_D12, n = 9), respectively. PBS as a control treatment (no YB1, n = 10). Lung metastasis was quantified on day 26. At each time point of the YB1 treatment, the status of lung metastasis was checked by H&E staining. b Representative H&E staining of lungs from mice killed on day 7 or 12 without YB1 treatment and those killed on day 26 with indicated treatments. c Quantification of 4T1 lung metastases (unpaired two-tailed t-tests) from mice as in a. d YB1 was performed on day 19 (YB1_D19, n = 10) after 4T1 implantation into BALB/c mice. PBS as a control treatment (No YB1, n = 9). Lung metastasis was quantified on day 28 (f). Extra mice were killed to check the status of lung metastasis by H&E staining on day 19 and 26 without YB1 treatment. e Representative H&E staining of lungs from indicated mice. f Quantification (unpaired two-tailed t-test) of 4T1 lung metastases on day 28. g, h Tracking and quantification of 4T1-Luci cells (two-sided multiple t-tests, p = 0.0163 (30 min), 0.0039 (40 min), 0.0024 (50 min), 0.0019 (60 min), 0.0015 (70 min), 0.0004 (90 min), 0.0023 (100 min), 0.0004 (110 min), 0.0002 (120 min), 0.0001 (130 min), and 0.00006 (140 min)) by luciferase live imaging after i.v. injection of 4T1-Luci into BALB/c mice pretreated with YB1 or PBS 3 days in advance (n = 5 per group). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. i Tracking and quantification (two-tailed unpaired t-test) of MB49-Luci cells by luciferase live imaging 3 h after i.v. injection of MB49-Luci into C57BL/6J mice pretreated with either YB1 or PBS 3 days in advance (n = 4 per group). b, e Red arrows indicate tumor tissues. Scale bar, 300 μm. c, f Displayed is a combined result of two independent experiments. g–i Displayed is one representative experiment of two independent experiments. All data are presented as mean values ± SEM. Source data are provided as a Source Data file.