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. 2021 May 5;12:2537. doi: 10.1038/s41467-021-22755-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Mice treatment timeline for CyTOF analysis. NOD SCID mice were divided into three groups treated with PBS, YB1 (YB1/IgG), or YB1 plus IFN-γ depletion antibody (YB1/anti-IFN-γ), respectively. All mice were killed on day 5 and lung-infiltrating immune cells were isolated and prepared for CyTOF analysis. b Representative t-SNE profile from each group of mice. Immune cell clusters 13, 14, 15, and 18 are highlighted by circles. c Percentage of each immune cell cluster per lung across samples (n = 4 per group). d Total cell number of each immune cell cluster per lung across samples (n = 4 per group). Immune cell clusters 13, 14, 15, and 18 were upregulated in the YB1 group compared to the other two control groups. c, d All data are presented as mean values ± SEM. e Characterization of each immune cell cluster. Based on the expressions of different phenotypic markers and cytokines, events acquired by CyTOF analysis of lung-infiltrating immune cells from NOD SCID mice with the three different treatments were merged for the t-SNE dimension reduction and PhenoGraph clustering analyses, and were divided into 18 immune cell clusters. Displayed one representative experiment of two independent experiments for all panels. Source data are provided as a Source Data file.