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. 2021 May 5;11:9588. doi: 10.1038/s41598-021-88887-0

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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3-D reconstructions of SFV-labelled OL and quantification of OL process morphology. (Ai) Maximum projection showing eGFP-f expression in a relatively simple white matter OL transduced with SFVA7(74). (Aii) 3-D reconstruction of the cell shown in (Ai). Segment colours (cyan, blue, green, yellow, gold, red, purple) indicate seven individual process arbors. Yellow circle indicates position of cell body. (Aiii) Reconstruction of the cell shown in (Ai) indicating position of four identified internodes (blue, purple, green, red). (Bi) Maximum projection showing eGFP-f expression in a relatively complex OL. (Bii) 3-D reconstruction for the cell shown in (Bi). Segment colours (blue, green, purple) indicate three individual process arbors. (Biii) Reconstruction of the cell shown in Bi indicating seven putative internodes (cyan, blue, green, yellow, gold, purple, red). Individual processes (green and purple in Bii) support multiple putative internodes. Inset: Expanded view of area indicated by dashed box reveals the initiation of internodes from neighboring internodes via fine connecting processes (white segments). Scale bars in (A) and (B) 20 µm. (C–H) Scatter plots displaying features of OL process morphology: mean process number per OL (7.67 ± 0.78) (C), mean process length (178 ± 37.5 µm) (D), average branch points per process (7.3 ± 1.05) (E), maximum branch order per process (7.25 ± 0.60) (F), mean internode number per cell 5.08 ± 0.45 (G), and mean internode length (83.7 ± 10.71 µm) (H). Arrows in (Ai)/(Aiii) and (Bi)/(Biii) indicate fine branches stemming from putative internodes. In (Aii–iii) and (Bii–iii) red and blue spheres indicate segment end-points and junction points respectively. Yellow circles indicate position of cell body. Data expressed as means ± SEM.