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. 2021 May 5;11:9588. doi: 10.1038/s41598-021-88887-0

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Complement mediated injury destroys internodes on SFV labelled OL. (A) Reconstruction of an SFV labelled OL expressing the CNPase-GFP transgene in a complement control treated slice. (Ai) mCherryMBP fluorescence reveals an OL with a typical myelinating morphology. (Aii) CNPase-GFP signals imaged from the field shown in (Ai) (white arrow indicates position of the mCherry⁺ OL). (Aiii) Merged image of the field shown in (Ai-ii) confirms co-localisation of mCherry (red) and CNPase-GFP (green) signals. (Aiv) 3-D reconstruction and analysis reveals 4 internodes (red) on the OL shown in (Ai–iii). (B) Morphological analysis of an A774 labelled OL following complement injury. (Bi) mCherry-f labelled OL with degenerating processes. (Bii) CNPase-GFP signals imaged from the field shown in (Bi). White arrow indicates position of mCherry⁺ OL shown in (Bi). (Biii) Merged image reveals localization of mCherry (red) and CNPase-GFP (green) signals. (Biv) Reconstruction and analysis of the cell shown in (Bi–iii) reveals fragmented processes (coloured segments) and the absence of internodes. Yellow circles indicate position of cell body. Scale bars in (A) and (B) 20 µm.