Figure 3.

(A) The Buffer subtracted ITC of EG1/ADPR yields K_d = 16 ± 4 nM (3 measurements) (B) nDSF signal (Ratio) for EG1/ADPR binding study with a protein concentration of 8 µM and ligand concentrations between 2 mM and 24 nM (14 dilutions). The region in the colored box was used for isothermal analysis (shown in D). (C) Melting temperature analysis with two different models yields apparent K_d,app values. (D) Isothermal analysis of nDSF data for ∆C_p = 0 at three selected temperatures. The same analysis for a fitted ∆C_p (cf. Table 1) is shown in Supplementary Information S25.