Figure 6.

(A) nDSF signal (fluorescence ratio) for Pcs60/γS-ATP titration. The region in the colored box was used for isothermal analysis (shown in C). (B) Melting temperature analysis with two different models yields apparent K_d,app values. (C) Isothermal analysis of nDSF data for ∆C_p = 0 at three selected temperatures. The same analysis for a fitted ∆C_p (cf. Table 1) is shown in supplementary information S28. (D) MST experiment with Pcs60/γS-ATP. The “cold” and “hot” regions used for calculating F_norm are marked as blue and red shadows, respectively. (E) Fitting F_norm to a 1:1 model yields a K_d of 16 µM.