Table 1.

Hourly expression of wakefulness (% Total Recording Time).

	Hour 1	Hour 2	Hour 3	Hour 4	Hour 5	Hour 6
Amiloride microinjected animals
Baseline	65.48 ± 7.82	31.68 ± 5.25	35.76 ± 4.21	35.76 ± 5.13	33.82 ± 4.75	38.50 ± 4.02
Vehicle	67.35 ± 6.05	41.64 ± 6.87	33.25 ± 4.15	35.57 ± 4.15	37.00 ± 8.17	36.00 ± 6.05
Low dose	50.19 ± 8.42	28.26 ± 4.71**	22.00 ± 2.82*	28.27 ± 7.85	29.59 ± 5.11	33.95 ± 4.62
High dose	50.32 ± 9.21	18.58 ± 6.26	26.72 ± 4.10	33.49 ± 6.12	34.01 ± 5.18*	23.94 ± 2.74**
APETx2 microinjected animals
Baseline	60.36 ± 7.019	19.67 ± 3.75	28.80 ± 5.34	36.79 ± 6.21	31.48 ± 4.91	34.03 ± 6.34
Vehicle	61.04 ± 8.05	22.22 ± 4.10	31.17 ± 4.15	36.16 ± 4.84	37.40 ± 7.74	41.76 ± 6.33
Low dose	50.70 ± 6.26	22.63 ± 5.87	19.34 ± 3.13*	31.28 ± 5.88	36.58 ± 3.24	27.45 ± 4.45**
High dose	40.04 ± 5.69	15.87 ± 2.11	17.17 ± 4.81*	28.27 ± 4.90	37.07 ± 6.61	37.36 ± 3.83

(*P < 0.05, **P < 0.01, one- way RM ANOVA followed by Tukey’s post hoc test).