Table 3.

Hourly expression of REM sleep (% TRT).

Amiloride	Hour 1	Hour 2	Hour 3	Hour 4	Hour 5	Hour 6
Amiloride microinjected animals
Baseline	1.80 ± 1.16	8.27 ± 3.28	9.20 ± 1.57	8.51 ± 1.58	8.60 ± 1.64	7.55 ± 1.90
Vehicle	1.56 ± 0.88	6.57 ± 1.24	9.46 ± 1.01	7.89 ± 1.73	8.99 ± 2.85	9.87 ± 2.34
Low dose	1.25 ± 0.71	2.16 ± 0.79	6.73 ± 1.55	6.19 ± 1.99	9.50 ± 2.81	8.13 ± 1.74
High dose	2.69 ± 1.36	4.66 ± 1.98	4.31 ± 1.51*	4.22 ± 1.01*	6.15 ± 1.19*	7.14 ± 1.64
APETx2 microinjected animals
Baseline	1.14 ± 0.57	9.85 ± 1.93	9.59 ± 1.98	10.28 ± 3.20	10.80 ± 1.38	5.93 ± 1.32
Vehicle	0.83 ± 0.43	8.59 ± 1.72	10.90 ± 2.08	10.15 ± 2.00	6.83 ± 2.05	6.78 ± 1.94
Low dose	0.57 ± 0.15	4.77 ± 1.37*	7.42 ± 2.00	6.00 ± 0.99*	4.97 ± 1.14**	4.59 ± 1.05
High dose	1.52 ± 0.66	4.19 ± 1.22*	4.65 ± 1.29*	5.25 ± 1.62	3.92 ± 1.12**	2.86 ± 1.07*

*P < 0.05, **P < 0.01, one-way RM-ANOVA followed by Tukey’s post hoc test.