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Seed virus and cell parameters for inoculation
| Viral tracer | Seed virus for inoculation | Host cell (Vero-E6) | ||||
|---|---|---|---|---|---|---|
| MOI | Virion # (pfu) | Tite(pfu/mL) | Vol. (mL) | Cell / flask | Flask # | |
| H129-G4 | 0.1 | 2.0 × 106 | 5.0 × 107 | 0.48 | 2.0 × 107 | 12 |
| H129-dTK-tdT | 0.1 | 2.0 × 106 | 5.0 × 106 | 4.80 | 2.0 × 107 | 12 |
| H129-dTK-T2 | 0.1 | 2.0 × 106 | 5.0 × 106 | 4.80 | 2.0 × 107 | 12 |
Vol. volume
