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. 2020 Dec 24;37(5):701–719. doi: 10.1007/s12264-020-00614-3

Table 4.

Injection parameters of H129-dTK-tdT monosynaptic tracer

Animal Brain region Helper AAV H129-dTK-tdT Perfusion time (day)b
AAVs (1 × 1012 vg/mL) Vol.a (μL) Inject. time Dose (pfu) Vol.a (μL) Inject. time For starter neuron For postsynaptic neuron
C57BL/6 MOB [16] AAV2/9-TK-GFP 0.45 Day 1 2.00 × 105 0.40 Day 22 Day 25 Day 29
C57BL/6 αBLA [22] AAV2/9-TK-GFP 0.20 Day 1 5.00 × 104 0.10 Day 22 Day 25 Day 27
C57BL/6 VPM [16] AAV2/9-TK-GFP 0.20 Day 1 1.00 × 105 0.20 Day 22 Day 25 Day 32
C57BL/6 LGN [16] AAV2/9-TK-GFP 0.20 Day 1 1.00 × 105 0.20 Day 22 Day 25 Day 32
C57BL/6 pBLA [22] AAV2/9-TK-GFP 0.20 Day 1 5.00 × 104 0.10 Day 22 Day 25 Day 27
PV-Cre nRT [16] AAV2/9-DIO-TK-GFP 0.35 Day 1 1.25 × 105 0.25 Day 22 Day 25 Day 32
DAT-Cre VTA [16] AAV2/9-DIO-TK-GFP 0.35 Day 1 1.25 × 105 0.25 Day 22 Day 25 Day 32

aInjected volume should not exceed 0.5 μL for brain regions.

bExtending the waiting time before perfusion normally results in higher labeling intensity and more labeled postsynaptic neurons. However, the starter neurons are also more severely damaged due to H129-dTK-tdT toxicity when conditional replication occurs.