Table 6.

Injection parameters of H129-dTK-T2 monosynaptic tracer

Animal	Brain region	Helper AAV			H129-dTK-T2			Perfusion time (Day)b
		AAV (1 × 1012 vg/mL)	Vol.a (μL)	Inject. time	Dose (pfu)	Vol.a (μL)	Inject. time
C57BL/6	MOB	AAV2/9-TK-GFP	0.45	Day 1	2.00 × 105	0.40	Day 22	Day 27
C57BL/6	DG	AAV2/9-TK-GFP	0.20	Day 1	1.00 × 105	0.20	Day 22	Day 27
C57BL/6	AuD	AAV2/9-TK-GFP	0.20	Day 1	5.00 × 104	0.10	Day 22	Day 28
C57BL/6	IC	AAV2/9-TK-GFP	0.20	Day 1	5.00 × 104	0.10	Day 22	Day 27

^aInjected volume should not exceed 0.5 μL for brain regions.

^bExtending the waiting time before perfusion normally results in brighter neural labeling and more labeled post-synaptic neurons. However, the starter neurons are also more severely damaged due to H129-dTK-T2 toxicity when replication occurs.