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. 2021 Apr 22;9:579906. doi: 10.3389/fbioe.2021.579906

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Copyright © 2021 Vargas Hoyos, Chiaramonte, Barbosa-Casteliani, Fernandez Morais, Perez-Jaramillo, Nobre Santos, Nascimento Queiroz and Soares Melo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HPLC analysis of organic acids involved in phosphorus solubilization. (A) Chromatographic profile of organic acid standards. (B) Extract of the 3AS4 strain obtained in phytate medium. (C) Extract of the 3AS4 strain obtained in calcium phosphate medium [Ca₃(PO₄)₂]. (D) Extract of the 3AS4 strain obtained in Rock Phosphate medium. All extracts were obtained after 12 days of growth in liquid medium under agitation (140 rpm) at 28°C. Numbers represent the different organic acids used, 1: oxalic acid, 2: citric acid, 3: gluconic acid, 4: 2-ketogluconic acid, 5: malonic acid, 6: succinic acid, 7: lactic acid, 8: formic acid, 9: malic acid, 10: propionic acid.