Table 1.
Semi-automated analysis procedure.
First method | Second method | Third method | Fourth method | |
---|---|---|---|---|
STEP 1 | Import the OCT scan into ImageJ | |||
STEP 2 | Set 8-bit format | |||
STEP 3 | Run A-trous-filter.java:
|
|||
STEP 4 | A Denoised image is obtained | |||
STEP 5 | Select main image | |||
STEP 6 | Process | |||
STEP 7 |
CLAHE (Enhance Local Contrast):
|
|||
STEP 8 | Plugin | |||
STEP 9 | Analyze | |||
STEP 10 |
Spot Counter:
|
Spot Counter:
|
Spot Counter:
|
Spot Counter:
|
STEP 11 | Free hand selection: | |||
• Select the area from RNFL/GCL to ELM | ||||
STEP 12 | Confirm the Spot Counter command | |||
STEP 13 | Copy the following values into a database: | |||
• n (whole number of spots counted) | ||||
• spot mean (average value of spot intensity) | ||||
• image mean (average value of image intensity) | ||||
• list of each single spot intensity. |