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. 2021 Apr 22;12:617042. doi: 10.3389/fimmu.2021.617042

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Copyright © 2021 Nunez Lopez, Casu and Pratley

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transmission electron micrographs of influenza virus A/Puerto Rico/8/34 H1N1 purified with steric exclusion chromatography (SXC) and pseudo-affinity chromatography with a sulfated cellulose membrane adsorber. Co-eluted exosome-like vesicles are visible in panels (A, B) and labeled with black arrows in panel (B). Panel (C) displays homogeneous (mostly intact) viral particles. Black arrows were added to the original published figure to highlight examples of extracellular vesicles (with lighter electron-density and collapsed cup shape) side by side with intact viral particles (non-collapsed round particles with higher electron density). All images are from the same sample at different magnifications. Pictures taken by Dietmar Riedel from the Max-Planck-Institute for Biophysical Chemistry in Göttingen, Germany. Reproduced with permission from Dr.-Ing. Pavel A Marichal-Gallardo, Max-Planck-Institute for Biophysical Chemistry in Göttingen, Germany (license CC BY-NC-SA 4.0).