Figure 5.

IL-4Rα signaling in cDCs or CD8α⁺DCs contributes to infiltration of immune cells, including pathogenic CD8⁺T cells, to the brain.A–D, the indicated mice (n = 6–7/group) were infected with 2 × 10⁵ PbA IRBCs. At 6 dpi, immune cells in the brains were isolated, stained with dye-conjugated antibodies, and analyzed by flow cytometry. A, cell gating strategy. B–E, the numbers of immune cells infiltrated into the brains; total immune cells (B), CXCR3⁺ CD4⁺ T and CD8⁺ T cells (C), granzyme B-expressing CD8⁺ T cells (D), and CD11a/LFA-1⁺ CD4⁺ T and CD8⁺ T cells. (E). Error bars indicate mean values ±SD. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001.