Figure 5.

Zurich variant knock-in heterozygous mouse (Col4a3^z/+mouse) shows pathologic phenotype in kidneys at 1 year of age. Histological analysis of periodic acid–Schiff (PAS)-stained kidney sections (×400) revealed varying degree of glomerular sclerosis (top left). Representative transmission electron microscopy (TEM) images of the glomerular capillary loops from two different Col4a3^z/+ mice and a wild-type control mouse are shown at the bottom. The glomerular basement membrane (GBM) was irregularly thickened in Col4a3^z/+ mice (red arrows; TEM images scale bars are 1 μm). One-year-old Col4a3^z/+ mice (n = 12) exhibited mild albuminuria compared with the age-matched WT mice (n = 9). Albumin-to-creatinine ratios are represented in graph (top right). Both experimental groups included male and female mice. The p value was 0.04.