Figure 2.

Diluted CSE also activates Ca²⁺ influx in hASMC. (A,C) Representative Ca²⁺ imaging traces from separate experiments tracking changes in [Ca²⁺]_i following perfusion with diluted CSE with or without extracellular Ca²⁺ (A), or following Ca²⁺ addback (1 mM Ca²⁺; black bar) after 5-min perfusion with 10% CSE in a nominally Ca²⁺-free solution (white bar), with or without 10-min pre-treatment with 100 µM Gd³⁺, which was also present throughout the experiment (C). (B,D) Summary of amplitude and rate of [Ca²⁺]_i changes corresponding to experiments in (A,C), respectively, presented as mean ± SEM. One-way ANOVA with Holm-Sidak’s multiple comparisons test was ran amongst the 3 groups (B; 3 independent donors; n = 9–14); unpaired t-test was performed between the control group and the Gd³⁺-treated group (D; 3 independent donors; n = 13–20). *p < 0.05. Control in (C,D) refers to CSE-activated Ca²⁺ addback without Gd³⁺ pre-treatment.