Figure 3.

CS-induced Ca²⁺ influx utilises a different pathway from SOCC and LTCC. (A,C) Representative Ca²⁺ imaging traces from separate experiments tracking changes in [Ca²⁺]_i following puffing one whole cigarette after 15-min treatment with 10 µM CPA (A), or after 10-min pre-treatment with 1 µM felodipine, which was also present throughout the experiment (C). Equal volumes of DMSO (0.01% for CPA, 0.001% for felodipine) were used for vehicle control. Experiments were run in the presence of 1 mM extracellular Ca²⁺ (black bar). (B,D) Summary of amplitude and rate of CS-induced [Ca²⁺]_i changes corresponding to experiments in (A,C), respectively, presented as mean ± SEM. Unpaired t-test was performed between the CPA-treated group (B; 2 independent donors; n = 4–5) or felodipine-treated group (D; 3 independent donors; n = 8–9) against the vehicle control group. *p < 0.05.