Figure 4.

CSE-induced Ca²⁺ influx also utilises a different pathway from SOCC and LTCC. (A,C) Representative Ca²⁺ imaging traces from separate experiments tracking changes in [Ca²⁺]_i following perfusion with 10% CSE after 15-min treatment with 10 µM CPA (A), or following Ca²⁺ addback (1 mM Ca²⁺; black bar) after 5-min perfusion with 10% CSE in a nominally Ca²⁺-free solution (white bar), with or without 10-min pre-treatment with 1 µM felodipine, which was also present throughout the experiment (C). Equal volumes of DMSO (0.01% for CPA, 0.001% for felodipine) were used for vehicle control. (B,D) Summary of amplitude and rate of CSE-induced or Ca²⁺ addback [Ca²⁺]_i changes corresponding to experiments in (A,C), respectively, presented as mean ± SEM. Unpaired t-test was performed between the CPA-treated group (B; 2 independent donors; n = 5–7) or felodipine-treated group (D; 4 independent donors; n = 19–23) against the vehicle control group. *p < 0.05.