Table 1.
Sequences of primers used in qPCR analysis. Primer pairs were designed using the NCBI Primer-BLAST tool.
| Primer | Sequence (5′ to 3′) | Product length | Position on mRNA/rRNA template |
|---|---|---|---|
| TRPA1 (forward) | GTG GAA CTT CAT ACC AGC TTA GA | 99 | 3113–3135 |
| TRPA1 (reverse) | AGA TCT GGG TTT GTT GGG ATA C | 3190–3211 | |
| GAPDH (forward) | TGC ACC ACC AAC TGC TTA GC | 87 | 476–495 |
| GAPDH (reverse) | GGC ATG GAC TGT GGT CAT GAG | 542–562 | |
| 18S (forward) | CTC TAG ATA ACC TCG GGC CG | 209 | 293–312 |
| 18S (reverse) | GTC GGG AGT GGG TAA TTT GC | 482–501 |