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. 2021 May 5;12:2538. doi: 10.1038/s41467-021-22764-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative example of flow cytometry analysis of ILC2-derived IL-13 upon co-culture with SW1116 CRC cells. b Frequencies of IL-13 and IL-5 positive ILC2s with T0070907 upon co-culture with SW1116 CRC cells ((Medium (open circle) Medium + SW1116 (open red circle) Medium + T0070907 + SW1116 (open blue circle)) (Medium n = 6, Medium+SW1116 n = 8, Medium + T0070907 + SW1116 n = 8; Medium vs Medium + SW1116 *P = 0.0313, Medium + SW1116 vs Medium + T0070907 + SW1116 *P = 0.0103). c Expression of IL-33 assessed by qPCR analysis in SW1116 CRC cells upon co-culture with ILC2s (SW1116 CTR (open circle) SW1116 + ILC2 (open red circle) SW1116 + ILC2 + T0070907 (open blue circle) (n = 3; *P = 0.0419). d Representative example of a wound healing assay (×20 magnification; Scale bar: 50 µm) performed using SW1116 CRC cells (CTR (open circle)) after incubation with ILC2 (open red circle) or ILC2 + T0070907 conditioned medium (CM) (open blue circle). e Quantification of the healed wound area at 24, 48, and 72 h (n = 5; **P = 0.0078, *P = 0.0493). f Representative example and quantification of clonogenic assays performed using SW1116 CRC cells (open circle) after incubation with ILC2 CM (open red circle), ILC2 + T0070907 CM (open blue circle) or ILC2 CM + anti-IL-13 blocking antibody (open green circle) for 14 days (SW1116 CTR n = 6, ILC2 CM and ILC2 + T0070907 CM n = 8, ILC2 CM + anti-IL13 n = 3; ***P = 0.0002, **P = 0.0085, *P = 0.0497). g Expression of MMP9 (left) and N-cadherin (right) assessed by qPCR analysis in SW1116 CRC cells (open circle) upon incubation with ILC2 CM (open red circle), ILC2 + T0070907 CM (open blue circle) or ILC2 CM + anti-IL-13 blocking antibody (open green circle) (SW1116 CTR, ILC2 CM, ILC2 + T0070907 CM n = 6, ILC2 CM + anti-IL13 n = 5; MMP9 SW1116 CTR vs ILC2 CM **P = 0.0035, ILC2 CM vs ILC2 + T0070907 CM *P = 0.0425, ILC2 CM vs ILC2 CM + anti-IL13 *P = 0.0152; NCAD SW1116 CTR vs ILC2 CM ****P < 0.0001, ILC2 CM vs ILC2 + T0070907 CM **P = 0.0041, ILC2 CM vs ILC2 CM + anti-IL13 ***P = 0.0002). Each symbol represents one individual donor or sample. Data are shown as mean ± SEM and were analyzed by one- (c, f, g) or two-way (b, e) ANOVA tests. Source data are provided as a Source data file.