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. 2021 May 5;12:2444. doi: 10.1038/s41467-021-22517-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Experimental design used to define the osteocyte transcriptome in each sex during skeletal maturation. b Representative micro-CT images of humeri from female and male mice at different ages. c The number of active genes and biotype composition of the osteocyte transcriptome at different ages in each sex. Any reflects the number of genes actively expressed in any ages or sexes, All reflects the percentage of genes active in any age or sex that are active in all ages and sexes. TEC = to be experimentally confirmed. d Principal component (PC) analysis of samples clustered by age in female (i) and male (ii) mice. The percentage of total variance explained by individual PCs is shown. Dots represent individual biological replicates and ellipses represent 50% confidence intervals for each age. Dist = Euclidean distance between cluster centroids (mean). P-values were calculated using Hotelling’s t-test (one-sided, Bonferroni adjusted), clusters with significant separation (P < 0.05) between centroids are highlighted in green. e Principal component analysis of samples clustered from both sexes at 4 (i), 10 (ii), 16 (iii) and 26 (iv) weeks of age. The percentage of total variance explained by individual PCs is shown. Dots represent individual biological replicates and ellipses represent 50% confidence intervals for each sex. P-values were calculated using Hotelling’s t-test (one-sided, Bonferroni adjusted), clusters with significant separation (P < 0.05) between centroids are highlighted in green.