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. 2021 May 5;12:2540. doi: 10.1038/s41467-021-22801-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Cellular composition analysis of cell type between patient groups for a neutrophils and b macrophage subtypes. Two-sided unpaired Wilcoxon test was performed to compare between groups for tests in a and b (LUSCn: n = 16, LUADn: n = 6, LUADm: n = 12 for both a and b. **p ≤ 0.01; *p ≤ 0.05; ns: p > 0.05). The lower hinge, middle line, and upper hinger of boxplots represented the first, second, and third quartiles of the distributions. The upper and lower whiskers corresponded to the largest and smallest data points within the 1.5 interquartile range. All actual data values were also plotted as dots alongside the boxplots. c Survival analysis for tissue-resident macrophage markers (MARCO and CD207) of LUAD and LUSC. d Correlation analysis between ITH_GEX and the cellular composition of patients. Only significantly associated cell types were shown. The tumor subtypes (LUAD, LUSC, and NSCLC) were shown in different colors and p-values were obtained by two-side t-tests (LUAD: n = 18, LUSC: n = 22, and NSCLC: n = 2).