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. 2021 Apr 22;15:670322. doi: 10.3389/fnins.2021.670322

FIGURE 2.

FIGURE 2

ETHR expression in the MB γ lobe is essential for courtship LTM performance. (A) Conditional knockdown of ETHR-encoding genes was achieved using the drug-inducible pan-neuronal GeneSwitch system (GSG301) by feeding RU486 for 3 days to day 4 males. LTM is lost in both GSG301/UAS-ETHR RNAi-Sym and GSG301/UAS-ETHR RNAi-IR2 males (n = 60–64). (B) ETHR knockdown using two independent RNAi lines in MB neurons sharply reduces MPI (n = 60–66). (C) Conditional ETHR silencing in adult MB neurons results in courtship LTM impairment (n = 64–72). (D,E) ETHR knockdown in MB γ-containing neurons impairs courtship LTM: (D) GAL4 lines utilized in this study: 201Y (αβ and γ), c739 (αβ), 4.59 (α′β′), and 1,471 (γ). Expression patterns were confirmed by crossing UAS-mCD8-GFP; (E) memory performances of MB lobe-specific ETHR-silenced males (n = 64–70). Random permutation test, *p < 0.05; **p < 0.01; and ***p < 0.001; ns, no significance.