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. 2021 Apr 22;12:592422. doi: 10.3389/fmicb.2021.592422

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Copyright © 2021 Greenman, Jurgensen, Holmes, Kapsak, Davis, Maza, Edemba, Esser, Hise, Keen, Larson, Lockwood, Wang, Harsh and Herrick.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Genetic map of the 184 kb IncA/C2 plasmid pHJ-38. Excised regions 1–5 of the plasmid contain mobility associated genes and genetic elements, antimicrobial and metal resistance genes, virulence genes, and other regions of interest. (B) Annotated gene regions containing heavy metal resistance genes (blue); virulence factors (light green); colicin genes (dark green); toxin-antitoxin gene cassettes (pink); genes associated with transposons, IS elements, or integrons (orange); and conjugation genes (black) are shown. Antimicrobial resistance genes identified by both Prokka and Abricate (purple) or by Prokka alone (red) are also shown. Yellow and cream-colored gene regions indicate hypothetical proteins. Blue lines indicate percent GC content.