Figure 5.

Time course of MI of viral epitope-specific CD8⁺ T cells in the lungs. (A) Gating strategy for the cytofluorometric quantitation of pulmonary CD8⁺ T cells expressing T-cell receptors specific for the pMHC-I complexes IE1-L^d, m164-D^d, and M105-K^d. Control, PE-conjugated pMHC-I dextramer H-2Kb/SIINFEKL. SSC, sideward scatter. Data refer to 6 months after HCT and infection. (B) Response kinetics of viral epitope-specific CD8⁺ T cells isolated from pulmonary infiltrates of the LL at the indicated times after HCT and infection. Note that, so far, antigenic peptides are not identified for proteins E1 and M86. Shown are median values and range for five mice per time of analysis. (C) Corresponding total numbers of transcriptional events as determined in SL, ML, IL, and PCL (recall Figure 4A ).