Figure 6.

Identification of iTEM as the “inflationary” subset of viral epitope-specific CD8⁺ T cells in the lungs. (A) Gating strategy for the cytofluorometric quantitation of viral epitope-specific KLRG1⁺CD62L^-CD127^- inflationary effector-memory cells (iTEM), KLRG1^-CD62L^-CD127^- early effector cells (EEC), KLRG1⁺CD62L^-CD127⁺ double-positive effector cells (DPEC), KLRG1^-CD62L^-CD127⁺ conventional effector-memory cells (cTEM), and KLRG1^-CD62L⁺CD127⁺ central memory cells (TCM). SSC, sideward-scatter. Data refer to IE1-specific cells measured at 6 months after HCT and infection. (B) Response kinetics of the indicated subsets of viral epitope-specific CD8⁺ T cells isolated from pulmonary infiltrates of the LL at the indicated times after HCT and infection. Shown are median values and range for five mice per time of analysis.