FIGURE 1.

Single-cell analysis in disease and health. Starting from the dissociation of target cells from the target tissue/organ, their isolation based on fluorescence-activated cell sorting (FACS) or other microfluidic techniques to RNA extraction. The RNA extraction is followed by cDNA synthesis by reverse transcriptase, followed by amplification and sequencing. From the sequencing, the reads are aligned and subjected to quantification that results in a quantification matrix or Gene Expression Matrix.