TABLE 3.

Commonly used methods for cell isolation on the bases of physical characteristics.

Technique	Mode of operation	Throughput	Advantages	Disadvantages	References
Microfluidic cell separation	Automatic	High	Works with low starting materials, amplification integration	High skills required, dissociated cells	Wyatt Shields et al., 2015
Micromanipulation manual cell picking	Manual	Low	More control over cell, live and intact cell separation	Laborious, high skills needed	Citri et al., 2012
Laser-capture microdissection	Manual	Low	Undamaged live cell capture, highly advanced	Too complex to operate, threat of contamination by neighboring cells	Espina et al., 2006
Density gradient centrifugation	Manual	Low	Cost-efficient	Too slow and laborious, low yield	Beakke, 1951