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. 2021 May 5;11:9587. doi: 10.1038/s41598-021-88983-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The combination of resminostat and sorafenib de-regulates the expression of EMT genes and decreases pERK level. (A) Relative mRNA expression of mesenchymal and cancer stem cell marker CD44 and CD133. PLT/PRF/5 cells were treated with or without platelets and with drug(s) as indicated. Data from three biological replicates are presented as data means with SD. Paired two-tailed t-test was performed. (B) Relative mRNA expression of EMT genes CDH2 (N-Cadherin), SNAI1, ZEB1, ID2, CDH1 (E-Cadherin), CLDN1, EpCAM and ITGA6. Data from three biological replicates are presented as data means with SD. Paired two-tailed t-test was performed. (C) Western blots for total level of the MEK signaling kinase ERK and its activated form pERK (phosphorylated ERK). GAPDH was used as loading control. PLC/PRF/5 cells were cultured with or without platelets and with drugs as indicated. Grouped blots were composed from cropped parts of individual blots for each antibody. Original blots with exposures and indicated cropped areas can be found in the supplementary information. (D) Same as in (C) but with SNU-475 cells. Staining for α-Tubulin was used as loading control.