Figure 3.

Ultrastructure of T. asahii CEMM 05-6-072 and T. inkin CEMM 05-6-074 biofilms by SEM. Sessile cells were formed on Thermanox™ coverslips in drug-free RPMI medium as controls (A, B). Mature biofilms (48 h) were challenged with 100 μg/mL AMB and incubated at 35°C for 24 h (C, D). Surviving cells were considered persisters. Biofilms were then scrapped with a pipette tip and inocula were prepared in RPMI medium. Progeny biofilms produced by persister cells (E, F) or mature biofilms-detached cells (G, H) were formed in RPMI medium.