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. 2021 Apr 22;11:645812. doi: 10.3389/fcimb.2021.645812

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Copyright © 2021 Cordeiro, Aguiar, Silva, Pereira, Portela, Camargo, Lima-Neto, Castelo-Branco, Rocha and Sidrim

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scanning electron microscopy of T. asahii CEMM 05-6-072 and T. inkin CEMM 05-6-074 biofilms. Sessile cells were formed on Thermanox™ coverslips in drug-free RPMI medium as controls (A, B). Mature biofilms (48 h) were challenged with 100 μg/mL AMB (C, D) for detection of persister cells. Progeny biofilms produced by persister cells (E, F) or mature biofilms-detached cells were formed in RPMI medium (G, H). Live cells are shown in green and dead/damaged cells are shown in yellow/red after stain with SYTO9 and propidium iodide, respectively. Magnification: 400x. Bar: 100 μm.