Table 2.

Minimum inhibitory concentration (MIC) of amphotericin B (AMB) against persister cells, biofilm cells and planktonic cells of T. asahii and T. inkin strains.

Species/Strains		AMB MIC (µg/mL)*
		Planktonic cells a	Biofilm cells b	Progeny biofilm c	Persister cells d	Progeny biofilm-persister cells e
T. asahii	CEMM 05-6-072	0.5	32	16	128	8
	CEMM 05-6-073	1	16	16	64	8
	CEMM 03-1-072	2	32	32	128	8
	CEMM 80	0.25	64	16	>128	2
	CEMM 81	0.5	64	32	>128	4
	Geometric mean	1.41	36.75	21.11	111.43	5
T. inkin	CEMM 01-1-143	0.5	16	8	>128	8
	CEMM 01-1-144	0.5	32	16	128	4
	CEMM 01-1-145	0.5	32	16	128	4
	CEMM 05-6-057	1	32	16	128	8
	CEMM 05-6-074	0.5	16	8	64	2
	CEMM 05-6-075	1	32	16	128	8
	CEMM 03-1-073	1	64	32	128	8
	Geometric mean	1	28.98	14.5	115.93	5

*100% inhibition of visible fungal growth.

^aObtained from 48-h cultures on PDA at 35°C.

^bDisaggregated cells from mature biofilms (48 h) produced by planktonic cells.

^cDisaggregated cells from progeny mature biofilms (48 h) produced by cells detached from mature biofilms.

^dPersister cells.

^eDisaggregated cells from progeny mature biofilms (48 h) produced by persister cells.