Figure 5.

GLYAT suppress breast cancer proliferation, metastasis and EMT in vivo. (A) Schematic diagram of the metastasis model in mice. (B–D) The mice injected with stable GLYAT KD MDA-MB-231 cells had markedly bigger and heavier tumors. (E–G) The mice injected with stable GLYAT OE MCF-7 cells had markedly smaller and lighter tumors. (H) Immunofluorescence assay of serial sections mouse tumor tissues revealed that GLYAT KD MDA-MB-231 cells significantly decreased the expression of E-cadherin whereas increased the expression of Vimentin and p-AKT. (I) Immunofluorescence assay of serial sections mouse tumor tissues revealed that, in GLYAT OE MCF-7 cells, the expression of E-cadherin was increased whereas Vimentin and p-AKT were decreased. (J) The schematic diagram of the role of GLYAT in BC. *p < 0.05, **p < 0.01. Scale bars are 50μm.