Figure 8.
Comparison of RV-C15- and RV-A1B-induced airway inflammation in house dust mite-sensitized and -challenged mice. Female 8-12 week-old BALB/c mice were challenged with house dust mite (HDM) and treated one day after the last HDM treatment with sham HeLa cell lysate, 5 x 106 ePFU RV-C15 or 5 x 106 ePFU RV-A1B. Forty-eight hrs later lungs were harvested for BAL analysis and qPCR. A separate set of mice were similarly treated and were anesthetized and endotracheally intubated for measurement of airways responsiveness. (A) Graphs showing BAL cell counts. (B) Graphs showing qPCR analysis of lung mRNA expression. The level of gene expression for each sample was normalized to GAPDH. For panels (A, B), data are mean ± SEM except for Ifng, n = 3-8 mice/group for 1-3 experiments, *p<0.05, one-way ANOVA; for Ifng, data are median ± IQR. (C) Airways methacholine responsiveness of the indicated treatment groups. Data are mean ± SEM of 3-4 mice/group from two experiments, *P < 0.05, two-way ANOVA.